Align a protein across species and score how conserved each residue is. Search UniProt or load FASTA; everything runs in your browser.
Add sequences
Type a gene name or accession. Orthologs are found through UniProt's OMA and OrthoDB groups, so orthologs with different gene names (PCNA is POL30 in budding yeast) are included.
Organism
Entry
Len
Plain or aligned FASTA. You can also drop files onto this box. UniProt headers are parsed for organism and gene.
Sequence set
Demo set. Ubiquitin from human, budding yeast and Arabidopsis, typed in to show the tool. Replace it with your own sequences before drawing conclusions.
Alignment and scoring settings
Add at least two sequences.
Conservation profile
variableconserved
Drag across the plot to zoom; double-click to reset. Click a position to jump to it in the alignment.
Alignment
Residue lookup
Positions use the reference sequence's numbering. A residue letter is optional and is checked against the reference.
Pairwise identity
% identical over positions where both sequences have a residue