Summary
Error bars and the default mean use one value per replicate (the replicate mean), so n is the number of replicates.
One symbol for every point, transparency marks the replicate, a line marks the mean.
Long format, one row per point: condition, replicate, value. Paste straight from Excel (tab-separated) or a CSV.
Error bars and the default mean use one value per replicate (the replicate mean), so n is the number of replicates.