Gate, compensate and summarise flow cytometry data in your browser
Add FCS files to start. Files are read on this computer and never uploaded, so the tool works offline and with unpublished data.
Drop FCS files or a .fwproj project here
- Compensation from the file’s spillover matrix, edited by hand, or computed from single-stain controls
- Transforms per channel: linear, log, arcsinh and logicle with automatic width
- Gates: rectangle, polygon, ellipse, interval and quadrant, editable after drawing
- Groups let one session hold several panels or donors, each with its own gating
- Projects save data and analysis in one file you can reopen or send to a colleague
- Figures export as PNG, vector SVG or PDF in six publication layouts
- Templates save the whole gating tree to apply to new files
Gating scheme
Compensation
Each row is a fluorochrome and each column a detector; values are percent spillover. Edits apply to every sample. Changes show immediately in the preview below.
Compute from single-stain controls
Assign each control file to the detector it is brightest in. The brightest 5% of events (excluding the top 0.2%) are compared with the unstained control, or with the dim half of the same control if none is given.
Preview
Active sample, all events. Left without compensation, right with the matrix above.
Preview
Statistics
Counts and frequencies for every population in every sample. Marker statistics use compensated, untransformed values.